Image-guided, Intravascular Hydrodynamic Gene Delivery to Skeletal Muscle in Pigs
نویسندگان
چکیده
منابع مشابه
Parameters Affecting Image-guided, Hydrodynamic Gene Delivery to Swine Liver
Development of a safe and effective method for gene delivery to hepatocytes is a critical step toward gene therapy for liver diseases. Here, we assessed the parameters for gene delivery to the livers of large animals (pigs, 40-65 kg) using an image-guided hydrodynamics-based procedure that involves image-guided catheter insertion into the lobular hepatic vein and hydrodynamic injection of repor...
متن کاملImage-Guided Hydrodynamic Gene Delivery: Current Status and Future Directions
Hydrodynamics-based delivery has been used as an experimental tool to express transgene in small animals. This in vivo gene transfer method is useful for functional analysis of genetic elements, therapeutic effect of oligonucleotides, and cancer cells to establish the metastatic cancer animal model for experimental research. Recent progress in the development of image-guided procedure for hydro...
متن کاملPolyplex nanomicelle promotes hydrodynamic gene introduction to skeletal muscle.
Skeletal muscle is an interesting target for gene therapy. To achieve effective gene introduction in skeletal muscle, a hydrodynamic approach by intravenous injection of plasmid DNA (pDNA) with transient isolation of the limb has attracted attention. In this study, we demonstrated that polyplex nanomicelle, composed of poly(ethyleneglycol) (PEG)-block-polycation and pDNA, showed excellent capac...
متن کاملAnimal Models for Hydrodynamic Gene Delivery
Hydrodynamic gene delivery (HGD) is an established method of the last decade where macromolecules, non-normally permeable to cell membrane, are delivered intracellular. The basic principle is that a large volume of solution, containing genes or oligonucleotides, RNA, proteins or other compounds, is infused rapidly in circulation to permit the entrance of these substances to parenchymal cells. E...
متن کاملGene expression in skeletal muscle.
Muscle has an intrinsic ability to change its mass and phenotype in response to activity. This process involves quantitative and qualitative changes in gene expression, including that of the myosin heavy chain isogenes that encode different types of molecular motors. This, and the differential expression of metabolic genes, results in altered fatigue resistance and power output. The regulation ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular Therapy
سال: 2010
ISSN: 1525-0016
DOI: 10.1038/mt.2009.206